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Catalog No: OKCA00994
Size:96 Wells
Price: $857.00
SKU
OKCA00994
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
1:59993-105
1:109390-97
1:209590-100
1:408480-88
ELISA Kit PrincipleAviva Systems Biology POMC ELISA Kit (Rat) (OKCA00994) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for POMC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POMC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample POMC captured in well.
ELISA Kit Range7.8 pg/mL-500 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9187-95
EDTA Plasma (n=4)8785-90
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level POMC were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level POMC were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
POMC Microplate96 Wells (12 x 8 Well strips)
POMC Lyophilized Standard2
100X Biotinylated POMC Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Culture Supernatant, Tissue Homogenates
Sensitivity1.95 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPOMC
Gene Full NamePro-opiomelanocortin
Protein NamePro-opiomelanocortin
Description of TargetACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
Uniprot IDP06297
Protein Accession #XP_008253036.1
Nucleotide Accession #XM_008254814.2
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