Size:96 Wells
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POMC ELISA Kit (Mouse) (OKEH03680)

Catalog#: OKEH03680
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology POMC ELISA Kit (Mouse) (OKEH03680) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Pomc antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pomc and incubated. The Pomc found in the sample or standards competes with the biotinylated Pomc for limited binding sites on the immobilized anti-human Pomc antibody. Excess unbound biotinylated Pomc and sample or standard Pomc is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pomc captured in the well and inversely proportional to the amount of Pomc which was contained in the sample or standard.
Kit Range 15.6 - 1000pg/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Kit Reproducibility Mean Intra-assay CV%: <6.3% (n = 20)
Mean Inter-assay CV%: <8.2% (n = 20)
Kit Component
Anti-Pomc Microplate96 Wells (12 x 8 Well strips)
Pomc Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Pomc-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 7.8 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Pro-opiomelanocortin
Assay Info Assay Methodology: Quantitative Competitive ELISA
Gene Symbol POMC
Official Gene Full Name pro-opiomelanocortin-alpha
Alias Symbols ACTH, adrenal corticotropic hormone, alpha-melanocyte stimulating hormone, alphaMSH, alpha-MSH, BE, beta-endorphin, beta-melanocyte stimulating hormone, beta-MSH, Corticotropin-lipotropin, gamma-melanocyte stimulating hormone, gamma-MSH, POMC, Pomc1, Pomc
NCBI Gene Id 18976
Protein Name Pro-opiomelanocortin
Description of Target ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.
Swissprot Id P01193
Protein Accession # NP_001265510.1
Nucleotide Accession # NM_001278581.1
Protein Size (# AA) 235
Molecular Weight 27 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Pomc.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Pomc.
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