Catalog No: OKEH03680
Size:96T
Price: $600.00
SKU
OKEH03680
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03680
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology POMC ELISA Kit (Mouse) (OKEH03680) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Pomc antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pomc and incubated. The Pomc found in the sample or standards competes with the biotinylated Pomc for limited binding sites on the immobilized anti-human Pomc antibody. Excess unbound biotinylated Pomc and sample or standard Pomc is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pomc captured in the well and inversely proportional to the amount of Pomc which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: <6.3% (n = 20)
Mean Inter-assay CV%: <8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Pomc Microplate96 Wells (12 x 8 Well strips)
Pomc Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Pomc-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse Pro-opiomelanocortin
Publications

Kim, Suhyeon, Ki-Bae Hong Singeun Kim, Hyung Joo Suh, Kyungae Jo. Creatine and taurine mixtures alleviate depressive-like behaviour in Drosophila melanogaster and mice via regulating Akt and ERK/BDNF pathways. Sci Rep. 2020 Jul 9 10(1):11370. 32647316

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPomc
Gene Full Namepro-opiomelanocortin-alpha
Alias SymbolsACT;ACTH;adrenal corticotropic hormone;adrenocorticotropic hormone;alp;alph;alpha-melanocyte stimulating hormone;alphaMSH;alpha-MSH;BE;beta-endorphin;Beta-LPH;beta-M;beta-MSH;Clip;corticotropin-like intermediary peptide;corticotropin-lipotropin;gamma-;Gamma-LPH;gamma-MSH;melanotropin gamma;met-enkephalin;Npp;PO;Pomc1;Pomc-1;pro-opiomelanocortin.
NCBI Gene Id18976
Protein NameP01193
Description of TargetPro-opiomelanocortin
Uniprot IDhttps://www.uniprot.org/uniprot/P01193
Protein Accession #NP_001265510.1
Nucleotide Accession #NM_001278581.1
Protein Size (# AA)235
Molecular Weight27 kDa
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