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Datasheets/Manuals | Printable datasheet for POLR3A Antibody (OACA02433) (OACA02433) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry |
Reconstitution and Storage | -20°C or -80°C |
Immunogen | Recombinant Human DNA-directed RNA polymerase III subunit RPC1 protein (392-632AA) |
Purification | Protein G Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Specificity | Human |
Formulation | Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300 |
Reference | [1] Novel compound heterozygous mutations of POLR3A revealed by whole-exome sequencing in a patient with hypomyelination. Shimojima K., Shimada S., Tamasaki A., Akaboshi S., Komoike Y., Saito A., Furukawa T., Yamamoto T. Brain Dev. 36:315-321 (2014). [2] Mutations in POLR3A and POLR3B are a major cause of hypomyelinating leukodystrophies with or without dental abnormalities and/or hypogonadotropic hypogonadism. Daoud H., Tetreault M., Gibson W., Guerrero K., Cohen A., Gburek-Augustat J., Synofzik M., Brais B., Stevens C.A., Sanchez-Carpintero R., Goizet C., Naidu S., Vanderver A., Bernard G. J. Med. Genet. 50:194-197 (2013). [3] Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy. Saitsu H., Osaka H., Sasaki M., Takanashi J., Hamada K., Yamashita A., Shibayama H., Shiina M., Kondo Y., Nishiyama K., Tsurusaki Y., Miyake N., Doi H., Ogata K., Inoue K., Matsumoto N. Am. J. Hum. Genet. 89:644-651 (2011). |
Gene Symbol | POLR3A |
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Gene Full Name | RNA polymerase III subunit A |
Alias Symbols | ADDH;C160;DNA-directed RNA polymerase III largest subunit;DNA-directed RNA polymerase III subunit A;DNA-directed RNA polymerase III subunit RPC1;HLD7;hRPC155;polymerase (RNA) III (DNA directed) polypeptide A, 155kDa;polymerase (RNA) III subunit A;RNA polymerase III 155 kDa subunit;RNA polymerase III subunit C1;RNA polymerase III subunit C160;RNA polymerase III subunit RPC155-D;RPC1;RPC155;WDRTS. |
NCBI Gene Id | 11128 |
Protein Name | DNA-directed RNA polymerase III subunit RPC1 |
Description of Target | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. |
Uniprot ID | O14802 |
Protein Accession # | NP_008986.2 |
Nucleotide Accession # | NM_007055.3 |
Protein Size (# AA) | 1390 |
Molecular Weight | 156 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "POLR3A Antibody (OACA02433)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "POLR3A Antibody (OACA02433)"?
This item is available "Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks".
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What buffer format is "POLR3A Antibody (OACA02433)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "POLR3A Antibody (OACA02433)"?
This target may also be called "ADDH;C160;DNA-directed RNA polymerase III largest subunit;DNA-directed RNA polymerase III subunit A;DNA-directed RNA polymerase III subunit RPC1;HLD7;hRPC155;polymerase (RNA) III (DNA directed) polypeptide A, 155kDa;polymerase (RNA) III subunit A;RNA polymerase III 155 kDa subunit;RNA polymerase III subunit C1;RNA polymerase III subunit C160;RNA polymerase III subunit RPC155-D;RPC1;RPC155;WDRTS." in publications.
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What is the shipping cost for "POLR3A Antibody (OACA02433)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "POLR3A Antibody (OACA02433)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "POLR3A Antibody (OACA02433)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "156 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "POLR3A Antibody (OACA02433)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "POLR3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "POLR3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "POLR3A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "POLR3A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "POLR3A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "POLR3A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.