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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OAAF00054-Biotin |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Biotin |
Application | WB, IHC, IF, ELISA |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The antiserum was produced against synthesized peptide derived between 1585-1634 amino acids from the C-Terminal region of human POLR2A around the phosphorylation site of Ser1619. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Specificity | POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1: 500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000 |
Reference | Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600 - 7612. Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833 - 843. Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161. |
Gene Symbol | POLR2A |
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Gene Full Name | DNA-directed RNA polymerase II subunit RPB1 |
Alias Symbols | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220 |
NCBI Gene Id | 5430 |
Protein Name | DNA-directed RNA polymerase II subunit RPB1 |
Uniprot ID | P24928 |
Protein Accession # | NP_000928.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
This target may also be called "RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220" in publications.
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What is the shipping cost for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "POLR2A Antibody (Phospho-Ser1619) : Biotin (OAAF00054-Biotin)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "POLR2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "POLR2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "POLR2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "POLR2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "POLR2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "POLR2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.