POLR2A Antibody (OASG06476)

Catalog No: OASG06476

Size:100 uL

Western Blot using HT29

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Monkey
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human Rpb1 around the non-phosphorylation site of S1619. Location: 1560-1640aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: Rpb1 Polyclonal Antibody detects endogenous levels of Rpb1 protein.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Target Info

• Gene Symbol: POLR2A
• Gene Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Alias Symbols: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220
• NCBI Gene Id: 5430; 20020;
• Description of Target: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
• Uniprot ID: P24928, P08775
• Molecular Weight: 250 kDa