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Datasheets/Manuals | Printable datasheet for POLR2A Antibody (OAAF00695) |
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Predicted Species Reactivity | Human|Monkey|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human POLR2A. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | POLR2A (Ab-1619) antibody detects endogenous levels of total POLR2A protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000 |
Reference | Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600 - 7612. Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833 - 843. Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161. |
Gene Symbol | POLR2A |
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Gene Full Name | RNA polymerase II subunit A |
Alias Symbols | DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase II subunit RPB1;DNA-directed RNA polymerase III largest subunit;hRPB220;hsRPB1;NEDHIB;POLR2;POLRA;polymerase (RNA) II (DNA directed) polypeptide A, 220kDa;polymerase (RNA) II subunit A;RNA polymerase II subunit B1;RNA-directed RNA polymerase II subunit RPB1;RPB1;RPBh1;RpIILS;RPO2;RPOL2. |
NCBI Gene Id | 5430 |
Protein Name | DNA-directed RNA polymerase II subunit RPB1 |
Description of Target | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity). |
Uniprot ID | P24928 |
Protein Accession # | NP_000928.1 |
Molecular Weight | 217 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "POLR2A Antibody (OAAF00695)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse|Rat".
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How long will it take to receive "POLR2A Antibody (OAAF00695)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "POLR2A Antibody (OAAF00695)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "POLR2A Antibody (OAAF00695)"?
This target may also be called "DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase II subunit RPB1;DNA-directed RNA polymerase III largest subunit;hRPB220;hsRPB1;NEDHIB;POLR2;POLRA;polymerase (RNA) II (DNA directed) polypeptide A, 220kDa;polymerase (RNA) II subunit A;RNA polymerase II subunit B1;RNA-directed RNA polymerase II subunit RPB1;RPB1;RPBh1;RpIILS;RPO2;RPOL2." in publications.
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What is the shipping cost for "POLR2A Antibody (OAAF00695)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "POLR2A Antibody (OAAF00695)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "POLR2A Antibody (OAAF00695)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "217 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "POLR2A Antibody (OAAF00695)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "POLR2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "POLR2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "POLR2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "POLR2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "POLR2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "POLR2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.