POLK ELISA Kit (Mouse) (OKEH07804)

Catalog No: OKEH07804

Size:96T

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology POLK ELISA Kit (Mouse) (OKEH07804) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POLK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POLK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POLK captured in well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.2% (n = 20)
  Mean Inter-assay CV%: <=8.2% (n = 20)
• ELISA Kit Component:
  • POLK Microplate: 96 Wells (12 x 8 Well strips)
  • POLK Lyophilized Standard: 2
  • 100X Biotinylated POLK Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
  Subcellular Location: Nucleus Detected throughout the nucleus and at replication foci.
  Unigene: Mm.89926
  SMR: Q9QUG2
  String: 10090.ENSMUSP00000022172
  KEGG: mmu:27015
  Pfam: PF00817
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 3.72pg/mL
• Specificity: Natural and recombinant Mouse DNA polymerase kappa
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: POLK
• Gene Full Name: polymerase (DNA directed), kappa
• Alias Symbols: Din, DINP, Dinb1
• NCBI Gene Id: 27015
• Protein Name: DNA polymerase kappa
• Uniprot ID: Q9QUG2
• Protein Accession #: NP_036178.1
• Nucleotide Accession #: NM_012048.3