POLD1 Antibody (OAGA00203)

Catalog No: OAGA00203

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA00203 diluted at 1:3000

DNA polymerase delta antibody detects POLD1 protein by Western blot analysis.
A. 30 ug Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (OAGA00203) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit(OAGA00203) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]
  Positive Controls: Molt-4, Rat2
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 685 and 1071 of DNA pol delta cat (Uniprot ID#P28340)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.56 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: POLD1
• Gene Full Name: DNA polymerase delta catalytic subunit
• Alias Symbols: CDC2, MDPL, POLD, CRCS10
• NCBI Gene Id: 5424
• Protein Name: DNA polymerase delta catalytic subunit
• Uniprot ID: P28340
• Protein Accession #: NP_001243778.1
• Molecular Weight: 124 kDa