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Catalog No: OKEH03248
Size:96T
Price: $525.00
SKU
OKEH03248
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PNOC ELISA Kit (Rat) (OKEH03248) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Pnoc antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pnoc and incubated. The Pnoc found in the sample or standards competes with the biotinylated Pnoc for limited binding sites on the immobilized anti-Rat Pnoc antibody. Excess unbound biotinylated Pnoc and sample or standard Pnoc is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pnoc captured in the well and inversely proportional to the amount of Pnoc which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Pnoc Microplate96 Wells (12 x 8 Well strips)
Pnoc Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Pnoc-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Rat Prepronociceptin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPnoc
Gene Full Nameprepronociceptin
Alias SymbolsN23K;neuropeptide nociceptin 1;nociceptin;Npnc1;orphanin FQ;prepronociceptin;Prepronociceptin (neuropeptide nociceptin) (N23K).
NCBI Gene Id25516
Protein NameQ62923
Description of TargetPrepronociceptin
Uniprot IDhttps://www.uniprot.org/uniprot/Q62923
Protein Accession #NP_037139.1
Nucleotide Accession #NM_013007.1
Protein Size (# AA)181
Molecular Weight20 kDa
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