Catalog No: OKCD08472
Size:96WELLS
Price: $800.00
SKU
OKCD08472
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Datasheets/ManualsPrintable datasheet for PNOC ELISA Kit (Human) (OKCD08472)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pronociceptin (PNOC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pronociceptin (PNOC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pronociceptin (PNOC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pronociceptin (PNOC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pronociceptin (PNOC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pronociceptin (PNOC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PNOC Microplate96 Wells (12 x 8 Well strips)
PNOC Lyophilized Standard2
100X PNOC HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.055ng/mL
Gene SymbolPNOC
Gene Full Nameprepronociceptin
Alias SymbolsN/OFQ;nociceptin;nocistatin;NOP;OFQ;orphanin FQ;ppN/OFQ;PPNOC;pre-pro-N/OFQ;prepronociceptin;pronociceptin.
NCBI Gene Id5368
Protein NamePrepronociceptin
Description of TargetNociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
Uniprot IDQ13519
Protein Accession #NP_001271173.1
Nucleotide Accession #NM_001284244.1
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