PNLIP ELISA Kit (Mouse) (OKCD02805)

Catalog No: OKCD02805

Size:96WELLS

Mouse PNLIP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-99%	80-101%	79-94%	97-105%
  EDTA Plasma (n=5)	86-101%	96-105%	92-101%	90-105%
  Heparin Plasma (n=5)	91-99%	78-96%	78-101%	80-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreatic Lipase (PL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreatic Lipase (PL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pancreatic Lipase (PL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pancreatic Lipase (PL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	99-105	102
  EDTA Plasma (n=5)	83-101	93
  Heparin Plasma(n=5)	92-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Lipase (PL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Lipase (PL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PNLIP Microplate: 96 Wells (12 x 8 Well strips)
  • PNLIP Lyophilized Standard: 2
  • 100X Biotinylated PNLIP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 2.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase, Pancreatic (PL). No significant cross-reactivity or interference between Lipase, Pancreatic (PL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pnlip
• Gene Full Name: pancreatic lipase
• Alias Symbols: 1810007A24Rik;P;pancreatic triacylglycerol lipase;pancreatic triglyceride lipase;PL;PTL.
• NCBI Gene Id: 69060
• Protein Name: Pancreatic triacylglycerol lipase
• Description of Target: Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones (By similarity).
• Uniprot ID: Q6P8U6
• Protein Accession #: NP_081201.2
• Nucleotide Accession #: NM_026925.3
• Protein Size (# AA): 465