PNLIP ELISA Kit (Human) (OKEH01028)

Catalog No: OKEH01028

Size:96T

Human PNLIP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PNLIP ELISA Kit (Human) (OKEH01028) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PNLIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PNLIP and incubated. The PNLIP found in the sample or standards competes with the biotinylated PNLIP for limited binding sites on the immobilized anti-human PNLIP antibody. Excess unbound biotinylated PNLIP and sample or standard PNLIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PNLIP captured in the well and inversely proportional to the amount of PNLIP which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • Anti-PNLIP Microplate: 96 Wells (12 x 8 Well strips)
  • PNLIP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PNLIP-Biotin Complex: 1 x 60 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 1.17U/L
• Specificity: Natural and recombinant Human Pancreatic triacylglycerol lipase
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PNLIP
• Gene Full Name: pancreatic lipase
• Alias Symbols: pancreatic triacylglycerol lipase;PL;PNLIPD;PTL;triacylglycerol acylhydrolase;triacylglycerol lipase.
• NCBI Gene Id: 5406
• Protein Name: P16233
• Description of Target: Pancreatic triacylglycerol lipase
• Uniprot ID: https://www.uniprot.org/uniprot/P16233
• Protein Accession #: NP_000927.1
• Nucleotide Accession #: NM_000936.2
• Protein Size (# AA): 465
• Molecular Weight: 51 kDa