PNCK Antibody - C-terminal region (OAAB16861)

Catalog No: OAAB16861

Size:400ul

Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB
• Additional Information: Precautions: PNCK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity)
  Cellular Location: Cytoplasm (By similarity). Nucleus (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human PNCK.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:10~50
  WB: 1:1000

Target Info

• Gene Symbol: PNCK
• Alias Symbols: BSTK3, CaMK1b
• NCBI Gene Id: 139728
• Protein Name: Calcium/calmodulin-dependent protein kinase type 1B
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q6P2M8
• Protein Accession #: NP_001034671.3
• Molecular Weight: 38500 Da