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Catalog No: OAAF01905 (Formerly GWB-ASD175)
Size:100 ug
Price: $344.00
SKU
OAAF01905
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsPrintable datasheet for PML Antibody (OAAF01905)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human PML.
PurificationThe antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Concentration1 mg/ml
SpecificityPML antibody detects endogenous levels of total PML protein.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoWB: 1:500~1000
IHC: 1:50~100
IF: 1:100~500
ELISA: 1:10000
ReferenceHitoshi Yoshida, Mol. Cell. Biol., Jun 2007; 10.1128.
Z M Mu, Mol. Cell. Biol., Oct 1994; 14: 6858 - 6867.
Alexander M. Ishov, J. Cell Biol., Oct 1999; 147: 221.
Zhi-Xiang Xu, Mol. Cell. Biol., Jun 2003; 23: 4247 - 4256.
Gene SymbolPML
Gene Full NamePML nuclear body scaffold
Alias SymbolsE3 SUMO-protein ligase PML;MYL;PML/RARA fusion;PP8675;probable transcription factor PML;promyelocytic leukemia protein;promyelocytic leukemia, inducer of;protein PML;RING finger protein 71;RING-type E3 SUMO transferase PML;RNF71;TRIM19;tripartite motif protein TRIM19;tripartite motif-containing protein 19.
NCBI Gene Id5371
Protein NameProtein PML
Description of TargetFunctions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function. Isoform PML-6 inhibits specifically the activity of the tetrameric form of PKM. The nuclear isoforms (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Isoform PML-2 is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration.
Uniprot IDP29590
Protein Accession #NP_002666.1
Molecular Weight97 kDa
  1. What is the species homology for "PML Antibody (OAAF01905)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "PML Antibody (OAAF01905)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "PML Antibody (OAAF01905)" provided in?

    This item is provided in "Liquid PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol ".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "PML Antibody (OAAF01905)"?

    This target may also be called "E3 SUMO-protein ligase PML;MYL;PML/RARA fusion;PP8675;probable transcription factor PML;promyelocytic leukemia protein;promyelocytic leukemia, inducer of;protein PML;RING finger protein 71;RING-type E3 SUMO transferase PML;RNF71;TRIM19;tripartite motif protein TRIM19;tripartite motif-containing protein 19." in publications.

  5. What is the shipping cost for "PML Antibody (OAAF01905)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "PML Antibody (OAAF01905)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "PML Antibody (OAAF01905)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "97 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "PML Antibody (OAAF01905)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "PML"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "PML"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "PML"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "PML"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "PML"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "PML"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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