PML Antibody - N-terminal region (OAGA04178)

Catalog No: OAGA04178

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
diluted at 1:5000

Immunoprecipitation of PML protein from A549 whole cell extracts using 5 ug of PML antibody [N1N2], N-term .
Western blot analysis was performed using PML antibody [N1N2], N-term .
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PML. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PML
• Gene Full Name: promyelocytic leukemia
• Alias Symbols: MYL, RNF71, PP8675, TRIM19
• NCBI Gene Id: 5371
• Protein Name: protein PML
• Description of Target: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Uniprot ID: P29590
• Protein Accession #: NP_002666.1
• Nucleotide Accession #: NM_002675.3