PMEL ELISA Kit (Human) : 96 Wells (OKEH01630)

Catalog No: OKEH01630

Size:96T

Human PMEL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PMEL ELISA Kit (Human) (OKEH01630) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PMEL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PMEL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PMEL captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - < 4.4%, n = 20
  Mean Inter-Assay Precision - < 7.8%, n = 20
• ELISA Kit Component:
  • PMEL Microplate: 96 Wells (12 x 8 Well strips)
  • PMEL Lyophilized Standard: 2
  • 100X Biotinylated PMEL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Melanocyte protein PMEL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PMEL
• Gene Full Name: premelanosome protein
• Alias Symbols: D12S53E;gp100;ME20;ME20M;ME20-M;melanocyte protein mel 17;melanocyte protein PMEL;melanocyte protein Pmel 17;melanocytes lineage-specific antigen GP100;melanoma-associated ME20 antigen;melanosomal matrix protein17;P1;P100;PMEL17;Premelanosome protein;SI;SIL;SILV;silver locus protein homolog;silver, mouse, homolog of.
• NCBI Gene Id: 6490
• Protein Name: P40967
• Description of Target: Melanocyte protein PMEL
• Uniprot ID: https://www.uniprot.org/uniprot/P40967
• Protein Accession #: NP_001186982.1
• Nucleotide Accession #: NM_001200053.1
• Protein Size (# AA): 661
• Molecular Weight: 70 kDa