PMAP23 ELISA Kit (Pig) (OKWB00337)

Datasheets/Manuals	Printable datasheet for PMAP23 ELISA Kit (Pig) (OKWB00337)

Predicted Species Reactivity

Porcine|Sus scrofa

Application

Enzyme-linked Immunosorbent assay-Sandwich

ELISA Kit Detection Method

Colorimetric, OD 450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Linearity

ELISA Kit Range

0.156-10ng/ml

ELISA Kit Reproducibility

Mean Intra-Assay Precision < 8%
Mean Inter-Assay Precision < 10%

ELISA Kit Component

Component	Amount
Pmap23 Microplate	96 Wells (12 x 8 Well strips)
Pmap23 Lyophilized Standard	2 x 10 ng
100X Pmap23 Biotinylated Detector Anitbody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Sensitivity

< 0.094 ng/mL

Publications

Role of Bacterial and Host DNases on Host-Pathogen Interaction during Streptococcus suis Meningitis. Int J Mol Sci. 21, NULL (2020). 32722502

Assay Info

Assay Type: Quantitative Sandwich ELISA

Description

Gene Symbol	PMAP-23
Gene Full Name	antibacterial protein
Alias Symbols	antibacterial peptide PMAP-23;cathelicidin;myeloid antibacterial peptide 23;PMAP23;PR-39.
NCBI Gene Id	100170138
Protein Name	Antibacterial peptide PMAP-23
Description of Target	Exerts antimicrobial activity against both Gram-positive and negative bacteria at concentrations of 2-16 micro molar. Its activity appears to be mediated by its ability to damage bacterial membranes.
Uniprot ID	P49930
Protein Accession #	NP_001123448
Nucleotide Accession #	NM_001129976

Protocol:

Tips Information:

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