Catalog No: OKBB00625
Size:96 Wells
Price: $876.00
SKU
OKBB00625
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||||||||||
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Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's mouse PLTP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards(NS0, E18-A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse PLTP amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 0.78 ng/ml - 50 ng/ml | ||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
Sample Type | Cell culture supernatants, serum, plasma (heparin, EDTA) | ||||||||||||||||||||||||||||||||||||||||||
Sensitivity | <20 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant mouse PLTP | ||||||||||||||||||||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: E18-A493 | ||||||||||||||||||||||||||||||||||||||||||
Description | For quantitative detection of mouse PLTP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Gene Symbol | PLTP |
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Gene Full Name | phospholipid transfer protein |
Alias Symbols | BPI fold containing family E, BPIFE, HDLCQ9, High absorbance lipoprotein cholesterol level quantitative trait locus 9, included, Lipid transfer protein II, OD107, Phospholipid transfer protein, Plasma phospholipid transfer protein, Pltp, PLTP_HUMAN |
NCBI Gene Id | 18830 |
Protein Name | Phospholipid transfer protein |
Description of Target | Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. |
Uniprot ID | P55065 |
Protein Accession # | NP_035255.1 |
Nucleotide Accession # | NM_011125.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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