Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKBB00625
Size:96 Wells
Price: $876.00
SKU
OKBB00625
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse PLTP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards(NS0, E18-A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse PLTP amount of sample captured in plate.
ELISA Kit Range0.78 ng/ml - 50 ng/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)9.418.52711.217.931
SD0.4980.9071.1340.750.9311.49
CV%5.34.94.26.75.24.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse PLTP Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse PLTP Standard2 x 50 ng/tube
100X Biotinylated Anti-Mouse PLTP Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum, plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant mouse PLTP
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: E18-A493
DescriptionFor quantitative detection of mouse PLTP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolPLTP
Gene Full Namephospholipid transfer protein
Alias SymbolsBPI fold containing family E, BPIFE, HDLCQ9, High absorbance lipoprotein cholesterol level quantitative trait locus 9, included, Lipid transfer protein II, OD107, Phospholipid transfer protein, Plasma phospholipid transfer protein, Pltp, PLTP_HUMAN
NCBI Gene Id18830
Protein NamePhospholipid transfer protein
Description of TargetPhospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
Uniprot IDP55065
Protein Accession #NP_035255.1
Nucleotide Accession #NM_011125.2
Write Your Own Review
You're reviewing:PLTP ELISA Kit (Mouse) (OKBB00625)
Your Rating
We found other products you might like!