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Catalog No: OKEH05078
Size:96 Wells
Price: $525.00
SKU
OKEH05078
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology PLPPR1 ELISA Kit (Human) (OKEH05078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLPPR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLPPR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLPPR1 captured in the well.
ELISA Kit Range0.312 - 20ng/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 102%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <7.2%, n = 20
Mean Inter-Assay Precision - <11.6%, n = 20
ELISA Kit Component
ComponentAmount
PLPPR1 Microplate96 Wells (12 x 8 Well strips)
PLPPR1 Lyophilized Standard2
100X Biotinylated PLPPR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.179 ng/mL
SpecificityNatural and recombinant Human Lipid phosphate phosphatase-related protein type 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPLPPR1
Gene Full NamePhospholipid phosphatase related 1
Alias SymbolsLPPR1, PRG-3
NCBI Gene Id54886
Protein NamePhospholipid phosphatase-related protein type 1
Description of TargetThis gene encodes a member of the plasticity-related gene (PRG) family. Members of the PRG family mediate lipid phosphate phosphatase activity in neurons and are known to be involved in neuronal plasticity. The protein encoded by this gene does not perform its function through enzymatic phospholipid degradation. This gene is strongly expressed in brain. It shows dynamic expression regulation during brain development and neuronal excitation. Alternatively spliced transcript variants encoding the same protein have been observed.
Uniprot IDQ8TBJ4
Protein Accession #NP_060223.2
Nucleotide Accession #NM_017753.2
Protein Size (# AA)325
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