PLP1 ELISA Kit (Human) : 96 Wells (OKEH00437)

Catalog No: OKEH00437

Size:96T

Human PLP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PLP1 ELISA Kit (Human) (OKEH00437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PLP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human PLP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PLP1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: <= 6.1%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 9.1% (n = 20)
• ELISA Kit Component:
  • Anti-PLP1 Microplate: 96 Wells (12 x 8 Well strips)
  • PLP1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated PLP1: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.072 ng/mL
• Specificity: Natural and recombinant Human Myelin proteolipid protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for PLP1 ELISA Kit (Human) : 96 Wells (OKEH00437) on CiteAb

Target Info

• Gene Symbol: PLP1
• Gene Full Name: proteolipid protein 1
• Alias Symbols: GPM6C;HLD1;lipophilin;major myelin proteolipid protein;MMPL;myelin proteolipid protein;PLP;PLP/DM20;PMD;SPG2.
• NCBI Gene Id: 5354
• Protein Name: P60201
• Description of Target: Myelin proteolipid protein
• Uniprot ID: https://www.uniprot.org/uniprot/P60201
• Protein Accession #: NP_000524.3
• Nucleotide Accession #: NM_000533.4
• Protein Size (# AA): 277
• Molecular Weight: 30 kDa