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Catalog No: OKCA01917
Size:96 Wells
Price: $754.00
SKU
OKCA01917
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology Plod1 ELISA Kit (Rat) (OKCA01917) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Plod1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Plod1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Plod1 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9388-96
EDTA Plasma (n=4)9895-102
ELISA Kit Component
ComponentAmount
Plod1 Microplate96 Wells (12 x 8 Well strips)
Plod1 Lyophilized Standard2 x 10 ng
100X Biotinylated Plod1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolPLOD1
Gene Full NameProcollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Alias SymbolsRP5-1077B9.2, FLJ42041, LH, LH1, LLH, PLOD, lysine hydroxylase|lysyl hydroxylase 1|procollagen-lysine, 2-oxoglutarate 5-dioxygenase
Protein NameProcollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Description of TargetForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Uniprot IDQ63321
Protein Accession #NP_446279.2
Nucleotide Accession #NM_053827.2
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