PLK1 ELISA Kit (Human) (OKEH04685)

Catalog No: OKEH04685

Size:96T

Human PLK1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology PLK1 ELISA Kit (Human) (OKEH04685) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PLK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLK1 captured in well.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - < 5.3%, n = 20
  Mean Inter-Assay Precision - < 8.9%, n = 20
• ELISA Kit Component:
  • Anti-PLK1 Microplate: 96 Wells (12 x 8 Well Strips)
  • PLK1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • PLK1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 3.4 pg/mL
• Specificity: Natural and recombinant Human Serine/threonine-protein kinase PLK1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PLK1
• Gene Full Name: polo like kinase 1
• Alias Symbols: cell cycle regulated protein kinase;PLK;PLK-1;polo (Drosophia)-like kinase;Polo-like kinase 1;serine/threonine-protein kinase 13;serine/threonine-protein kinase PLK1;STPK13.
• NCBI Gene Id: 5347
• Protein Name: P53350
• Description of Target: Serine/threonine-protein kinase PLK1
• Uniprot ID: https://www.uniprot.org/uniprot/P53350
• Protein Accession #: NP_005021.2
• Nucleotide Accession #: NM_005030.5
• Protein Size (# AA): 603
• Molecular Weight: 68 KDa