PLIN2 ELISA Kit (Rat) (OKCA02585)

Catalog No: OKCA02585

Size:96 Wells

Rat PLIN2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	85-94
  1:2	102	96-105
  1:4	92	87-98
  1:8	94	89-98

• ELISA Kit Principle: Aviva Systems Biology PLIN2 ELISA Kit (Rat) (OKCA02585) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLIN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLIN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard PLIN2 captured in the well.
• ELISA Kit Range: 15.6 - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	98	93-102
  EDTA Plasma (n=4)	103	92-107

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • PLIN2 Microplate: 96 Wells (12 x 8 Well strips)
  • PLIN2 Lyophilized Standard: 2
  • 100X Biotinylated PLIN2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PLIN2
• Alias Symbols: ADFP
• NCBI Gene Id: 280981
• Protein Name: Perilipin-2
• Description of Target: May be involved in development and maintenance of adipose tissue.
• Uniprot ID: Q9TUM6
• Protein Accession #: NP_776405
• Nucleotide Accession #: NM_173980