PLGF ELISA Kit (Human) (OKBB00242)

Catalog No: OKBB00242

Size:96 Tests

Human PGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards(E.coli, A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human PLGF amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	52	146	526	45	165	583
  St. Dev.	3.28	8.47	39.5	3.24	10.9	49
  %CV	6.30	5.80	7.50	7.2	6.60	8.40

• ELISA Kit Component:
  • Lyophilized recombinant human MMP-7 standard: 10 ng/tube x 2
  • Anti-human MMP-7 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human MMP-7 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Principle: Aviva’s human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human PLGF specific polyclonal antibody was pre-coated onto 96-well plates. The human specific detection polyclonal antibody was biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLGF amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Maglione, D.; Guerriero, V.; Viglietto, G.; Delli-Bovi, P.; Persico, M. G. : Isolation of a human placenta cDNA coding
  for a protein related to the vascular permeability factor. Proc. Nat. Acad. Sci. 88: 9267-9271, 1991.
  2. Autiero, M.; Waltenberger, J.; Communi, D.; Kranz, A.; Moons, L.; Lambrechts, D.; Kroll, J.; Plaisance, S.; De Mol,
  M.; Bono, F.; Kliche, S.; Fellbrich, G.; and 16 others : Role of PlGF in the intra- and intermolecular cross talk
  between the VEGF receptors Flt1 and Flk1. Nature Med. 9: 936-943, 2003.
  3. PLGF stimulated angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency
  to VEGF. Luttun, A.; Tjwa, M.; Moons, L.; Wu, Y.; Angelillo-Scherrer, A.; Liao, F.; Nagy, J. A.; Hooper, A.; Priller, J.;
  De Klerck, B.; Compernolle, V.; Daci, E.; and 10 others : Revascularization of ischemic tissues by PlGF treatment,
  and inhibition of tumor angiogenesis, arthritis and atherosclerosis by anti-Flt1. Nature Med. 8: 831-840, 2002.
• Specificity: Natural and recombinant human PLGF
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A21-R149
• Description: For quantitative detection of human PLGF in cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine.

Target Info

• Gene Symbol: PGF
• Gene Full Name: placental growth factor
• Alias Symbols: AI854365, D12S1900, MGC93298, Pgf, PGFL, PIGF, Placenta growth factor, Placental growth factor, Placental growth factor vascular endothelial growth factor related protein, PlGF, PlGF 2, PLGF_HUMAN, PlGF2, SHGC 10760, Vascular endothelial growth factor related protein
• NCBI Gene Id: 5228
• Protein Name: Placenta growth factor
• Description of Target: The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
• Uniprot ID: P49763
• Protein Accession #: NP_001193941.1
• Nucleotide Accession #: NM_001207012.1