PLG Chemi-Luminescent ELISA Kit (Human) (OKCD03886)

Catalog No: OKCD03886

Size:96WELLS

Human PLG ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-104%	80-101%	86-101%	85-96%
  EDTA Plasma (n=5)	86-94%	80-101%	78-101%	95-104%
  Heparin Plasma (n=5)	79-94%	94-102%	90-97%	80-102%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen (Plg). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen (Plg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen (Plg) level in the sample or standard.
• ELISA Kit Range: 0.41-300ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-93	88
  EDTA Plasma (n=5)	95-105	101
  Heparin Plasma (n=5)	91-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen (Plg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen (Plg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PLG Microplate: 96 Wells (12 x 8 Well strips)
  • PLG Lyophilized Standard: 2
  • 100X PLG Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.41 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PLG
• Gene Full Name: plasminogen
• Alias Symbols: HAE4;plasmin;plasminogen.
• NCBI Gene Id: 5340
• Protein Name: Plasminogen
• Description of Target: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
• Uniprot ID: P00747
• Protein Accession #: NP_000292
• Nucleotide Accession #: NM_000301