Catalog No: OPPA00710 (Formerly GWB-C3B242)
Size:5UG
Price: $75.00
SKU
OPPA00710
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA00710 |
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Predicted Species Reactivity | Human |
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Product Format | Sterile filtered and lyophilized from 0.5 mg/ml in 0.1M phosphate buffer and 0.1M NaCl, pH 7.2. |
Host | E. Coli |
Application | ELISA, WB |
Additional Information | Solubility: Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely. |
:: | Product Introduction: Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24% of the residues); lysine cluster sequences are found in the N-terminal and C-terminal ends of the structure.The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblast-enriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pleiotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages. Product Description: Pleiotrophin Human Recombinant contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3 kDa protein containing 136 amino acid residues of the OSF-1 human and 16 additional amino acid residues - HisTag, thrombin cleavage site (underlined).MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD. |
Reconstitution and Storage | Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C. |
Purity | Greater than 95% as determined by SDS-PAGE. Purification: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. |
Specificity | The amino acid sequence of the Pleiotrophin human recombinant is 100% homologous to the amino acid sequence of the OSF-1 human without signal sequence. |
Gene Symbol | Pleiotrophin |
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Alias Symbols | HARP, HBBM, HBNF, HBGF8, NEGF1, OSF-1, HB-GAM, HBGF-8, HBNF-1 |
NCBI Gene Id | 5764 |
Protein Name | Pleiotrophin |
Description of Target | Recombinant Human Pleiotrophin |
Uniprot ID | P21246 |
Protein Accession # | NP_002816.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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