Catalog No: OKIA00208
Size:1 x 96 well plate
Price: $1,150.00
SKU
OKIA00208
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Hamster | ||||||||||||||||||
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Product Format | 12 of 8-well strips | ||||||||||||||||||
Application | ELISA | ||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||
ELISA Kit Duration | 170 Minutes | ||||||||||||||||||
ELISA Kit Principle | The Aviva Systems Biology PLBL2 ELISA Kit (Hamster) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Hamster (CHO) Phospholipase B-like in Tissue Culture Fluid. This assay employs an antibody specific for Hamster (CHO) PLBL2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Phospholipase B-like present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-PLBL2 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLBL2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of PLBL2 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution. | ||||||||||||||||||
ELISA Kit Range | 0.31 - 20 ng/mL | ||||||||||||||||||
ELISA Kit Reproducibility | < 10% | ||||||||||||||||||
ELISA Kit Component |
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Additional Information | Intended Use: The PLBL2 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring PLBL2/Phospholipase B-like in Hamster (CHO) biological samples. | ||||||||||||||||||
:: | Sample Volume: 15 uL | ||||||||||||||||||
Reconstitution and Storage | Store at 4 degrees | ||||||||||||||||||
Sensitivity | 0.06 ng/mL |
Alias Symbols | PLBL2;Phospholipase B-like; |
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Protein Name | Phospholipase B-like |
Description of Target | Putative phospholipase, PLBL2 has been associated as a host cell protein contaminant in CHO antibody development projects with potential for interference in human immune responses. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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