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Catalog No: OKIA00208
Size:1 x 96 well plate
Price: $1,150.00
SKU
OKIA00208
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHamster
Product Format12 of 8-well strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration170 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology PLBL2 ELISA Kit (Hamster) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Hamster (CHO) Phospholipase B-like in Tissue Culture Fluid. This assay employs an antibody specific for Hamster (CHO) PLBL2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Phospholipase B-like present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-PLBL2 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLBL2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of PLBL2 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range0.31 - 20 ng/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
ComponentAmount
Anti-Hamster PLBL2 Microplate96 Wells (12 x 8 Well Strips)
Hamster PLBL2 Calibrator1
100X PLBL2 Detection Antibody150 uL
100X Streptavidin-HRP Conjugate150 uL
1X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The PLBL2 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring PLBL2/Phospholipase B-like in Hamster (CHO) biological samples.
::Sample Volume: 15 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity0.06 ng/mL
Alias SymbolsPLBL2;Phospholipase B-like;
Protein NamePhospholipase B-like
Description of TargetPutative phospholipase, PLBL2 has been associated as a host cell protein contaminant in CHO antibody development projects with potential for interference in human immune responses.
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