PLAUR ELISA Kit (Human) (OKEH04009)

Catalog No: OKEH04009

Size:96T

Human PLAUR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PLAUR ELISA Kit (Human) (OKEH04009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLAUR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLAUR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLAUR captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.4% (n = 20)
  Mean Inter-assay CV%: <= 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-PLAUR Microplate: 96 Wells (12 x 8 Well Strips)
  • PLAUR Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • PLAUR Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.022 ng/mL
• Specificity: Natural and recombinant Human Urokinase plasminogen activator surface receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PLAUR
• Gene Full Name: plasminogen activator, urokinase receptor
• Alias Symbols: CD87;monocyte activation antigen Mo3;UPAR;U-PAR;u-plasminogen activator receptor form 2;URKR;urokinase plasminogen activator surface receptor;urokinase-type plasminogen activator (uPA) receptor.
• NCBI Gene Id: 5329
• Protein Name: Q03405
• Description of Target: Urokinase plasminogen activator surface receptor
• Uniprot ID: https://www.uniprot.org/uniprot/Q03405
• Protein Accession #: NP_001005376.1
• Nucleotide Accession #: NM_001005376.2
• Protein Size (# AA): 335
• Molecular Weight: 37 kDa