PLAU ELISA Kit (Pig) (OKCD02972)

Catalog No: OKCD02972

Size:96WELLS

Pig PLAU ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-101%	86-102%	99-105%	99-105%
  EDTA Plasma (n=5)	83-97%	98-105%	97-104%	87-96%
  Heparin Plasma (n=5)	78-93%	95-102%	80-101%	88-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator, Urokinase (uPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator, Urokinase (uPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator, Urokinase (uPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Plasminogen Activator, Urokinase (uPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-99	84
  EDTA Plasma (n=5)	80-95	84
  Heparin Plasma(n=5)	80-92	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PLAU Microplate: 96 Wells (12 x 8 Well strips)
  • PLAU Lyophilized Standard: 2
  • 100X Biotinylated PLAU Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator, Urokinase (uPA). No significant cross-reactivity or interference between Plasminogen Activator, Urokinase (uPA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PLAU
• Gene Full Name: plasminogen activator, urokinase
• Alias Symbols: uPA;U-plasminogen activator;urinary plasminogen activator;urokinase-type plasminogen activator.
• NCBI Gene Id: 396985
• Protein Name: Urokinase-type plasminogen activator
• Description of Target: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
• Uniprot ID: P04185
• Protein Accession #: NP_999110.1
• Nucleotide Accession #: NM_213945.1
• Protein Size (# AA): 442