PLAU Antibody - N-terminal region (OAAB18604)

Catalog No: OAAB18604

Size:400ul

Western blot analysis of anti-PLAU Antibody (N-term) (Cat. #OAAB18604) in mouse brain tissue lysates (35ug/lane).
PLAU (arrow) was detected using the purified Pab.
Dilution: 1:1000

Western blot analysis of anti-PLAU Antibody (N-term) (Cat. #OAAB18604) in A2058 cell line lysates (35ug/lane).
PLAU (arrow) was detected using the purified Pab.
Dilution: 1:1000

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PLAU antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Dilution: 1:10~50

Urokinase (PLAU) Antibody (N-term) (Cat. #OAAB18604) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Dilution: 1:10~50

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Urokinase (PLAU) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human Urokinase (PLAU).
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Reference: Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Sperl, S., et al., Proc. Natl. Acad. Sci. U.S.A. 97(10):5113-5118 (2000). Turkmen, B., et al., Electrophoresis 18(5):686-689 (1997). Conne, B., et al., Thromb. Haemost. 77(3):434-435 (1997). Yoshimoto, M., et al., Biochim. Biophys. Acta 1293(1):83-89 (1996).

Target Info

• Gene Symbol: PLAU
• Alias Symbols: ATF, QPD, UPA, URK, u-PA, BDPLT5
• NCBI Gene Id: 5328
• Description of Target: PLAU, a member of the peptidase family S1, is a potent plasminogen activator and is clinically used for therapy of thrombolytic disorders. PLAU specifically cleaves the Arg-|-Val bond in plasminogen to form plasmin. The protein is found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A. Cleavage occurs after residue 155 in the low molecular mass form to yield a short A1 chain. The protein is used in Pulmonary Embolism (PE) to initiates fibrinolysis. Structurally, PLAU contains 1 EGF-like domain and 1 kringle domain.
• Uniprot ID: P00749
• Protein Accession #: NP_001138503.1, NP_002649.1
• Molecular Weight: 49 kDa