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Catalog No: OKIA00129 (Formerly GWB-344550)
Size:1x96WELLPLATE
Price: $509.00
SKU
OKIA00129
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration90 Minutes
ELISA Kit PrincipleAviva Systems Biology Plasminogen ELISA Kit (Rabbit) (OKIA00129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Plasminogen has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Plasminogen - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plasminogen captured in the well.
ELISA Kit Range6.25 ng/ml - 200 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rabbit Plasminogen Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rabbit Plasminogen Calibrator1
Additional InformationIntended Use: The Plasminogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of rabbit.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rabbit Plasminogen antibody conjugated
with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RABBIT PLASMINOGEN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rabbit PMG.
7. RABBIT PLASMINOGEN CALIBRATOR
One vial containing Rabbit Plasminogen calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.905 ng/ml
Predicted Homology Based on Immunogen SequenceRabbit
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protein NamePlasminogen
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