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Catalog No: OKIA00080 (Formerly GWB-91D15C)
Size:1x96WELLPLATE
Price: $575.00
SKU
OKIA00080
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration130 Minutes
ELISA Kit PrincipleAviva Systems Biology Plasminogen ELISA Kit (Human) (OKIA00080) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Plasminogen has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Plasminogen - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plasminogen captured in the well.
ELISA Kit Range6.25 ng/ml - 200 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human Plasminogen Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Plasminogen Calibrator1
Additional InformationIntended Use: The Plasminogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of human.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Plasminogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN PLASMINOGEN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human PMG.
7. HUMAN PLASMINOGEN CALIBRATOR
One vial containing a lyophilized Human Plasminogen calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.114 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPLG
Gene Full Nameplasminogen
Alias SymbolsPlasminogen, PLG, Plasmin, Plasmin light chain B
NCBI Gene Id5340
Protein NamePlasminogen
Description of TargetPlasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Uniprot IDP00747
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