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Catalog No: OKCD03722
Size:96WELLS
Price: $800.00
SKU
OKCD03722
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PLA2G7 Chemi-Luminescent ELISA Kit (Human) (OKCD03722)

Datasheets/ManualsPrintable datasheet for PLA2G7 Chemi-Luminescent ELISA Kit (Human) (OKCD03722)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-94%78-95%91-105%81-92%
EDTA Plasma (n=5)78-92%80-95%98-105%90-104%
Heparin Plasma (n=5)87-94%90-104%92-101%79-90%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Group VII (LpPLA2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Phospholipase A2 Group VII (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phospholipase A2 Group VII (LpPLA2) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-10491
EDTA Plasma (n=5)95-10299
Heparin Plasma (n=5)85-10594
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2 Group VII (LpPLA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2 Group VII (LpPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PLA2G7 Microplate96 Wells (12 x 8 Well strips)
PLA2G7 Lyophilized Standard2
100X PLA2G7 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPLA2G7
Gene Full Namephospholipase A2 group VII
Alias Symbols1-alkyl-2-acetylglycerophosphocholine esterase;2-acetyl-1-alkylglycerophosphocholine esterase;group-VIIA phospholipase A2;gVIIA-PLA2;LDL-associated phospholipase A2;LDL-PLA(2);LDL-PLA2;lipoprotein-associated phospholipase A2;LP-PLA2;PAF 2-acylhydrolase;PAF acetylhydrolase;PAFAD;PAFAH;phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma);platelet-activating factor acetylhydrolase.
NCBI Gene Id7941
Protein NamePlatelet-activating factor acetylhydrolase
Description of TargetModulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Uniprot IDQ13093
Protein Accession #NP_001161829
Nucleotide Accession #NM_001168357
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