PLA2G6 ELISA Kit (Rat) (OKCD00797)

Catalog No: OKCD00797 (Formerly GWB-KBBDH1)

Size:96WELLS

Rat Pla2g6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-105%	94-103%	79-99%	78-97%
  EDTA plasma(n=5)	85-97%	80-99%	80-94%	89-103%
  heparin plasma(n=5)	88-96%	96-104%	78-89%	79-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Calcium Independent (iPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase A2, Calcium Independent (iPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-99	95
  EDTA plasma(n=5)	99-105	102
  heparin plasma(n=5)	78-94	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pla2g6 Microplate: 96 Wells (12 x 8 Well Strips)
  • Pla2g6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Pla2g6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Calcium Independent (iPLA2). No significant cross-reactivity or interference between Phospholipase A2, Calcium Independent (iPLA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Pla2g6
• Gene Full Name: phospholipase A2 group VI
• Alias Symbols: 2-lysophosphatidylcholine acylhydrolase;85 kDa calcium-independent phospholipase A2;85/88 kDa calcium-independent phospholipase A2;caI-PLA2;Group VI phospholipase A2;GVI PLA2;intracellular membrane-associated calcium-independent phospholipase A2 beta;iPla2;iPLA2-beta;palmitoyl-CoA hydrolase;patatin-like phospholipase domain-containing protein 9;phospholipase A2, group VI (cytosolic, calcium-independent);PNPLA9.
• NCBI Gene Id: 360426
• Protein Name: 85/88 kDa calcium-independent phospholipase A2
• Description of Target: Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods (By similarity).
• Uniprot ID: P97570
• Protein Accession #: NP_001005560
• Nucleotide Accession #: NM_001005560.1
• Protein Size (# AA): 807