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Catalog No: OKCD09374
Size:96WELLS
Price: $750.00
SKU
OKCD09374
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Datasheets/ManualsPrintable datasheet for PLA2G4D ELISA Kit (Human) (OKCD09374)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IVD (PLA2G4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipase A2, Group IVD (PLA2G4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Group IVD (PLA2G4D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Group IVD (PLA2G4D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PLA2G4D Microplate96 Wells (12 x 8 Well strips)
PLA2G4D Lyophilized Standard2
100X PLA2G4D HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.064ng/mL
Gene SymbolPLA2G4D
Gene Full Namephospholipase A2 group IVD
Alias SymbolscPLA2delta;cPLA2-delta;cytosolic phospholipase A2 delta;phospholipase A2 delta, cytosolic;Phospholipase A2 group IVD;phospholipase A2, group IVD (cytosolic).
NCBI Gene Id283748
Protein NameCytosolic phospholipase A2 delta
Description of TargetCalcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position (PubMed:14709560). May have a substrate specificity for linoleic acid at the sn-2 position (PubMed:14709560).
Uniprot IDQ86XP0
Protein Accession #NP_828848.3
Nucleotide Accession #NM_178034.3
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