Catalog No: OKEH03386
Size:96T
Price: $525.00
SKU
OKEH03386
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PLA2G4A ELISA Kit (Rat) (OKEH03386) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pla2g4a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pla2g4a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pla2g4a captured in well.
ELISA Kit Range3.12-200U/mL
ELISA Kit Component
ComponentAmount
Anti-Pla2g4a Microplate96 Wells (12 x 8 Well strips)
Pla2g4a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Pla2g4a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity1.56U/mL
SpecificityNatural and recombinant Rat Cytosolic phospholipase A2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPLA2G4A
Gene Full Namephospholipase A2 group IVA
Alias SymbolsPhospholipase A2 group IVA.
NCBI Gene Id24653
Protein NameP50393
Description of TargetCytosolic phospholipase A2
Uniprot IDhttps://www.uniprot.org/uniprot/P50393
Protein Accession #NP_598235.2
Nucleotide Accession #NM_133551.2
Protein Size (# AA)752
Molecular Weight86 kDa
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