PLA2G2A ELISA Kit (Rat) (OKEH07110)

Catalog No: OKEH07110

Size:96T

Rat PLA2G2A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Pla2g2a ELISA Kit (Rat) (OKEH07110) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pla2g2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pla2g2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pla2g2a captured in the well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 90%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <5.6%
  Inter-Assay CV <9.1%
• ELISA Kit Component:
  • Pla2g2a Microplate: 96 Wells (12 x 8 Well strips)
  • Pla2g2a Lyophilized Standard: 2 x 50 ng
  • 100X Pla2g2a HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.406 ng/mL
• Specificity: Natural and recombinant Rat Phospholipase A2, membrane associated
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pla2g2a
• Gene Full Name: phospholipase A2 group IIA
• Alias Symbols: eted enzyme type IIA phospholipase A2;GIIC sPLA2;group IIA phospholipase A2;phosphatidylcholine 2-acylhydrolase 2A;phospholipase A2, group IIA (platelets, synovial fluid);phospholipase A2, membrane associated;platelet phospholipase A2;sPLA2.
• NCBI Gene Id: 29692
• Protein Name: P14423
• Description of Target: Phospholipase A2, membrane associated
• Uniprot ID: https://www.uniprot.org/uniprot/P14423
• Protein Accession #: NP_113786
• Nucleotide Accession #: NM_031598