PL 12 Protein (OPPA00825)

Catalog No: OPPA00825 (Formerly GWB-95174A)

Size:20UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid 16mM HEPES buffer (pH 8), 250mM sodium chloride, 20% glycerol
• Host: Sf9 Insect Cells
• Application: WB
  Immunological Functions: 1. Binds IgG-type human auto-antibodies.
  2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
  Coating Concentration: 0.3-0.8 ug/mL (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Reconstitution and Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Purification: Purified by proprietary chromatographic techniques.
• Concentration: 1 mg/ml
• Purity: Greater than 90% as determined by SDS-PAGE.
• Tag: 6xHis tag

Target Info

• Gene Symbol: PL 12
• Alias Symbols: AARS, CMT2N, DEE29, EIEE29
• NCBI Gene Id: 16
• Protein Name: Alanine--tRNA ligase, cytoplasmic
• Description of Target: Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop. AARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain.
• Uniprot ID: P49588
• Protein Accession #: NP_001596.2
• Protein Size (# AA): Recombinant
• Molecular Weight: 108 kDa