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Catalog No: OKCD00803 (Formerly GWB-KBBDV1)
Size:96WELLS
Price: $650.00
SKU
OKCD00803
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Datasheets/ManualsPrintable datasheet for PKN1 ELISA Kit (Human) (OKCD00803)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PKN1 Microplate96 Wells (12 x 8 Well Strips)
PKN1 Lyophilized Standard2 x 20 ng
100X Biotinylated PKN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase N1 (PKN1). No significant cross-reactivity or interference between Protein Kinase N1 (PKN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPKN1
Gene Full Nameprotein kinase N1
Alias SymbolsDBK;PAK1;PAK-1;PKN;PKN-ALPHA;PRK1;PRKCL1;protease-activated kinase 1;protein kinase C-like 1;protein kinase C-like PKN;protein kinase C-related kinase 1;protein kinase PKN-alpha;Protein-kinase C-related kinase 1;serine/threonine protein kinase N;serine/threonine-protein kinase N1;serine-threonine kinase N;Serine-threonine protein kinase N.
NCBI Gene Id5585
Protein NameSerine/threonine-protein kinase N1
Description of TargetPKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Uniprot IDQ16512
Protein Accession #NP_002732
Nucleotide Accession #NM_002741.3
Protein Size (# AA)942
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