Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEI00203
Size:96 Wells
Price: $625.00
SKU
OKEI00203
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PKIA ELISA Kit (Human) (OKEI00203) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PKIA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PKIA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample PKIA
ELISA Kit Range0.313 - 20 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level PKIA. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level PKIA using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
LACTB2 Microplate96 Wells (12 x 8 Well strips)
LACTB2 Lyophilized Standard2
100X LACTB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPKIA
Alias SymbolsPRKACN1
NCBI Gene Id5569
Protein NamecAMP-dependent protein kinase inhibitor alpha
Description of TargetExtremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
Uniprot IDP61925
Protein Accession #NP_006814
Nucleotide Accession #NM_006823
Write Your Own Review
You're reviewing:PKIA ELISA Kit (Human) (OKEI00203)
Your Rating
We found other products you might like!