PKC nu Antibody - C-terminal region (OAAB16759)

Catalog No: OAAB16759

Size:400ul

Western blot analysis of anti-PKCnu C-term Pab (Cat. #OAAB16759) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (OAAB16759) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and OAAB16759 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: PKC nu Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity)
  Cellular Location: Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation
  Tissue Location: Ubiquitous.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-890 amino acids from the C-terminal region of human PKC nu.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PRKD3 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PRKD3
• Alias Symbols: EPK2, PKD3, PRKCN, PKC-NU, nPKC-NU
• NCBI Gene Id: 23683
• Protein Name: Serine/threonine-protein kinase D3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O94806
• Protein Accession #: NP_005804.1
• Molecular Weight: 100471 Da