PKC eta Antibody - N-terminal region (OAAB16752)

Catalog No: OAAB16752

Size:400ul

Western blot analysis of anti-PKC eta N-term Pab (Cat. # OAAB16752) in NCI-H460 cell lysate. PKC eta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of PRKCH (arrow) using PKC eta Antibody (N-term) (Cat.# OAAB16752).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCH gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Cellular Location: Cytoplasm
  Tissue Location: Most abundant in lung, less in heart and skin.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PKC eta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the N-terminal region of human PKC eta.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 1.2 mg/ml
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: PRKCH
• Alias Symbols: PKCL, PKC-L, PRKCL, nPKC-eta
• NCBI Gene Id: 5583
• Protein Name: Protein kinase C eta type
• Description of Target: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC eta is one of the PKC family members. This protein is most abundant in lung, less in heart and skin.
• Uniprot ID: P24723
• Protein Accession #: NP_006246.2
• Molecular Weight: 78 kDa