PKCb Antibody (Phospho-Thr641) (OAEC00165)

Catalog No: OAEC00165

Size:50ug

Western blot analysis of extracts from K562 cells, untreated or treated with PMA (1ng/ml, 10min), using PKCβ (Ab-641) antibody (OAEC00526, Lane 1 and 2) and PKCβ (phospho-Thr641) antibody (OAEC00165, Lane 3 and 4).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCβ (phospho-Thr641) antibody (OAEC00165).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, IHC, WB
• Reconstitution and Storage: Store at -20C/1 year
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKCβ around the phosphorylation site of threonine 641 (E-L-T^P-P-T).
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
• Concentration: 1 mg/ml
• Specificity: PKCβ (phospho-Thr641) antibody detects endogenous levels of PKCβ only when phosphorylated at threonine 641.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:500-1:1000, IHC: 1:50-1:100
• Reference: Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761 Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653 Marcil J, et al. (1999) Biochem J ; 337:185-192 Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.

Target Info

• Gene Symbol: PRKCB
• Gene Full Name: Protein kinase C beta type
• Alias Symbols: PKCB, PRKCB1, PRKCB2, PKCI(2), PKCbeta, PKC-beta
• NCBI Gene Id: 5579
• Protein Name: Protein kinase C beta type
• Uniprot ID: P05771
• Protein Accession #: NP_002729.2
• Molecular Weight: 77 kDa