Catalog No: OPPA01120 (Formerly GWB-86FD71)
Size:5UG
Price: $835.00
SKU
OPPA01120
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA01120 |
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Predicted Species Reactivity | Human |
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Product Format | PKAkt1 1.9mg/ml, in 50mM NaCl, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol, pH 8.5. Physical appearance: Sterile Filtered clear solution. |
Host | Sf9 Insect Cells |
:: | Product Introduction: Akt1, also known as "Akt" or protein kinaseB (PKB) is an important molecule in mammaliancellular signaling.In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These enzymesare members of the serine/threonine-specific protein kinasefamily (EC2.7.11.1).Akt1 is involved in cellular survival pathways, by inhibiting apoptoticprocesses. Akt1 is also able to induce protein synthesispathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogenein the transforming retrovirus, AKT8. Product Description: Recombinant Human Protein Kinase B is a glycosylated polypeptide having a molecular mass of 59.1 kDa. Recombinant Protein Kinase B is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles. Unit Definition20. 000 Units/mg (1 Unit = 1 pmol/min transferred to synthetic peptide RPRAATF at 30Celsius). No protease activity detectable. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Gene Symbol | PKAkt1/PKBa |
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Alias Symbols | AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA |
NCBI Gene Id | 207 |
Protein Name | RAC-alpha serine/threonine-protein kinase |
Description of Target | Recombinant Human Protein Kinase Akt1/PKB alpha Active Enzyme |
Uniprot ID | P31749 |
Protein Accession # | NP_001014431.1 |
Protein Size (# AA) | Active|Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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