PIWI-L2 Antibody - N-terminal (OAPB01502)

Catalog No: OAPB01502

Size:100UG

Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL.

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse|Rat
• Product Format: Liquid. PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410 - 460 of PIWI-L2.
• Purification: Affinity Purified
• Concentration: 1 mg/ml

Target Info

• Gene Symbol: PIWIL2
• Gene Full Name: piwi like RNA-mediated gene silencing 2
• Alias Symbols: cancer/testis antigen 80;CT80;HILI;mili;PIWIL1L;piwil2-like protein;piwi-like 2;piwi-like protein 2;testicular tissue protein Li 141.
• NCBI Gene Id: 55124
• Protein Name: Piwi-like protein 2
• Description of Target: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (By similarity). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660).
• Uniprot ID: Q8TC59
• Protein Accession #: NP_00129193
• Nucleotide Accession #: NM_001135721.1