PISD ELISA Kit (Bovine) (OKWB00100)

Catalog No: OKWB00100

Size:96T

Bovine PISD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-100%	85-104%	86-101%	86-104%
  EDTA Plasma (n=5)	84-101%	82-95%	88-93%	83-97%
  Heparin Plasma (n=5)	81-88%	83-90%	83-97%	88-99%

• ELISA Kit Principle: Aviva Systems Biology Pisd ELISA Kit (Bovine) (OKWB00100) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pisd has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pisd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pisd captured in the well.
• ELISA Kit Range: 31.25-2000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	88-105	98
  EDTA Plasma (n=5)	87-101	96
  Heparin Plasma (n=5)	95-105	99

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Pisd Microplate: 96 Wells (12 x 8 Well strips)
  • Pisd Lyophilized Standard: 2 x 2 ng
  • 100X Pisd Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 18.75 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PISD
• Gene Full Name: phosphatidylserine decarboxylase
• Alias Symbols: phosphatidylserine decarboxylase proenzyme, mitochondrial.
• NCBI Gene Id: 505332
• Protein Name: Phosphatidylserine decarboxylase proenzyme, mitochondrial
• Description of Target: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.
• Uniprot ID: Q58DH2
• Protein Accession #: NP_001019646
• Nucleotide Accession #: NM_001024475