PIR ELISA Kit (Mouse) (OKEI00506)

Catalog No: OKEI00506

Size:96 Wells

Mouse PIR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Pir ELISA Kit (Mouse) (OKEI00506) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pir has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pir is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Pir
• ELISA Kit Range: 0.781 - 50 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Pir. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Pir using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • LACTB2 Microplate: 96 Wells (12 x 8 Well strips)
  • LACTB2 Lyophilized Standard: 2
  • 100X LACTB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 0.469 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PIR
• Alias Symbols: Pirnl, 2310042L19Rik
• NCBI Gene Id: 69656
• Protein Name: Pirin
• Description of Target: Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).
• Uniprot ID: Q9D711
• Protein Accession #: NP_081429
• Nucleotide Accession #: NM_027153