PIR Antibody (OAGA04368)

Catalog No: OAGA04368

Size:100 ul

Pirin antibody detects PIR protein by Western blot analysis.
A. 50 ug mouse liver lysate/extract
12 % SDS-PAGE
Pirin antibody dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
Pirin antibody
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded MPG xenograft, using Pirin antibody at 1:500 dilution.

Pirin antibody detects Pirin protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Pirin antibody dilution: 1:500.

Pirin antibody detects Pirin protein at cytosol and nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
Pirin antibody dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pirin antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pirin. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.96 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PIR
• Gene Full Name: pirin
• NCBI Gene Id: 8544
• Protein Name: pirin
• Description of Target: This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.
• Uniprot ID: O00625
• Protein Accession #: NP_001018119.1
• Nucleotide Accession #: NM_001018109.2