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Datasheets/Manuals | Printable datasheet for PIR Antibody (OAEB01173) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Additional Information | Reactivity Info: Tested: Human; Expected: Human, Mouse |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | PIR |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100% |
Peptide Sequence | GSSKKVTLSVLSREC |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 ug/ml. |
Reference | * Wendler WM, Kremmer E, Forster R, Winnacker EL.; Identification of pirin, a novel highly conserved nuclear protein.; J Biol Chem. 1997 Mar 28;272(13):8482-9.; Pubmed 9079676 |
Gene Symbol | PIR |
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Gene Full Name | pirin |
Alias Symbols | pirin;pirin (iron-binding nuclear protein);probable quercetin 2,3-dioxygenase PIR;probable quercetinase. |
NCBI Gene Id | 8544 |
Protein Name | Pirin |
Uniprot ID | O00625 |
Protein Accession # | NP_003653.1 |
Molecular Weight | 32.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PIR Antibody (OAEB01173)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "PIR Antibody (OAEB01173)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PIR Antibody (OAEB01173)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PIR Antibody (OAEB01173)"?
This target may also be called "pirin;pirin (iron-binding nuclear protein);probable quercetin 2,3-dioxygenase PIR;probable quercetinase." in publications.
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What is the shipping cost for "PIR Antibody (OAEB01173)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PIR Antibody (OAEB01173)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PIR Antibody (OAEB01173)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "32.1".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PIR Antibody (OAEB01173)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PIR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PIR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PIR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PIR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PIR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PIR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.