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Catalog No: OKEH01799
Size:96T
Price: $625.00
SKU
OKEH01799
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PIP4K2C ELISA Kit (Human) (OKEH01799) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIP4K2C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIP4K2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIP4K2C captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
PIP4K2C Microplate96 Wells (12 x 8 Well strips)
PIP4K2C Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PIP4K2C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Human Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPIP4K2C
Gene Full Namephosphatidylinositol-5-phosphate 4-kinase type 2 gamma
Alias Symbolsphosphatidylinositol 4-phosphate 5-kinase;phosphatidylinositol 5-phosphate 4-kinase type II gamma;phosphatidylinositol 5-phosphate 4-kinase type-2 gamma;phosphatidylinositol-4-phosphate 5-kinase, type II, gamma;PI(5)P 4-kinase type II gamma;PIP4KII-gamma;PIP5K2C.
NCBI Gene Id79837
Protein NameQ8TBX8
Description of TargetPhosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Uniprot IDhttps://www.uniprot.org/uniprot/Q8TBX8
Protein Accession #NP_001139730.1
Nucleotide Accession #NM_001146258.1
Protein Size (# AA)421
Molecular Weight47 kDa
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